Embedded Files
Handling iTru primers in plates (1.25 nmol / well; from us).
Making iTru Y-yoke adapter stubs (5nmol aliquots; from us).
Making iTru libraries with Kapa reagents.
Making iNext libraries with NEB & Kapa reagents.
Dual-digest RADseq libraries with quadruple indexing.
How to create double-stranded adapters from oligos (primers) that you order yourself (not from us).
How to make, use, and validate Speedbeads.
Dual-digest RADseq libraries with PCR duplicate tagging.
Dual-digest RADseq adapters - 4 x 2 designs.
Dual-digest RADseq libraries with quadruple indexing.
Making iTru libraries for PCR products with NEB reagents.
How to dissolve iTru blocking primers to make adapter blockers.
How to create double-stranded adapters from normalized oligo pairs (what we send out).
UCE Protocols
UCE Protocols
This was a big deal at the time, but you can now do even better with Kapa Hyper Prep Kits.
UCE Sequence Capture with Universal Blockers
Again, this was helpful at the time, but you can now do better by using the latest MYbaits-manual from Arbor Biosciences (formerly Mycroarray).
Post-enrichment limited cycle PCR
This can be good to have, but you can simply use the latest MYbaits-manual from Arbor Biosciences (formerly Mycroarray).
Validations of enrichments via qPCR
This is a reasonable way to test if new baits are doing what you want before you spend a lot on sequencing. However, now that we have so many iTru primers, it will usually be cheaper and better to accomplish this task via low depth sequencing.
Generic_iTru5_iTru7_Plate_Map.xlsxGeneric iTru Primer Plate Layouts
Click here to download the Generic plate layouts of iTru primers.
iTru_Full_Sequences_June2016.xlsiTru Primer Sequences (192 + 387)
Click here to download the excel file with 192 iTru5 and 387 iTru7 primers from the Adapterama I on bioRxiv in 2016.
3RAD Adapter Plate Maps
Click here to download the maps for 3RAD adapter plates.
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